Anti-PI3-kinase p85 subunit alpha antibody [A3-D0]

Anti-PI3-kinase p85 subunit alpha antibody [A3-D0]

• 概述

• 产品描述Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of (p85) and (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and γ, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.
• 产品名称Anti-PI3-kinase p85 subunit alpha antibody [A3-D0]
• 分子量84kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol, Membrane, Nucleus
• 其它名称
  • GRB1 antibody
  • p85 alpha antibody
  • p85 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control:
  Lane 1: U-937
  Lane 2: Raji

   

  

  Fig2: Immunocytochemical staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig3: Immunocytochemical staining of MCF-7 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig4: Immunocytochemical staining of SH-SY-5Y cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig5: Immunocytochemical staining of PANC-1 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig7: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig8: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.

   

  

  Fig9: Flow cytometric analysis of MCF-7 cells with PI3-kinase p85 subunit alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG wa

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