Anti-Eftud2 antibody [1-D10-A1]

Anti-Eftud2 antibody [1-D10-A1]

• 概述

• 产品描述Spliceosomes are multi-protein complexes that are composed of snRNPs (small nuclear ribonucleoproteins) and a variety of associated protein factors, all of which work in concert to regulate the splicing of pre-mRNA. Snrp116, also known as EFTUD2 (elongation factor Tu GTP binding domain containing 2) or Snu114, is a 972 amino acid protein that localizes to the nucleus and belongs to the GTP-binding elongation factor family. Existing as a component of the multi-protein U5 snRNP spliceosome complex, Snrp116 plays an important role in pre-mRNA splicing, as well as in the recycling of spliceosomal snRNPs. The gene encoding Snrp116 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
• 产品名称Anti-Eftud2 antibody [1-D10-A1]
• 分子量116 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus
• 其它名称
  • 116 kDa antibody
  • 116 kDa U5 small nuclear ribonucleoprotein component antibody
  • EFTUD2 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis on different cell lysates using anti-EFTUD2 Mouse mAb. Positive control:
  Lane 1: Jurkat
  Lane 2: NIH/3T3

   

  

  Fig2: Immunocytochemical staining of SH-SY-5Y cells using anti-EFTUD2 Mouse mAb

   

  

  Fig3: Immunocytochemical staining of SW480 cells using anti-EFTUD2 Mouse mAb.

   

  

  Fig4: Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-EFTUD2 Mouse mAb.

   

  

  Fig5: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-EFTUD2 Mouse mAb.

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-EFTUD2 Mouse mAb.

   

  

  Fig7: Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-EFTUD2 Mouse mAb.

   

  

  Fig8: Flow cytometric analysis of MCF-7 cells with EFTUD2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without ncubation with primary antibody; black). Goat anti mouse IgG (FITC) was used as the secondary antibody.

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