Anti-CD137 antibody [2G1]

Anti-CD137 antibody [2G1]

• 概述

• 产品描述CD137, also designated ILA and 4-1BB in mouse, belongs to the tumor necrosis factor receptor family and delivers a costimulatory signal to T lymphocytes. CD137 is expressed on activated T cells and binds an inducible ligand that is found on B cells, macrophages, and dendritic cells. Interactions between CD137 and its ligand are involved in antigen presentation and the generation of cytotoxic T cells. Crosslinking of the CD137 ligand induces apoptosis in resting lymphocytes. In contrast, CD137 regulates peripheral monocyte survival by inducing a cytokine release profile, and is mediated by M-CSF and to a lesser extent by granulocyte-macrophage colony-stimulating factor and IL-3. Soluble forms of CD137 are found in sera from patients with rheumatoid arthritis and may provide a negative control mechanism for immune responses.
• 产品名称Anti-CD137 antibody [2G1]
• 分子量35 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane. Extracellular space.
• 其它名称
  • 4 1BB antibody
  • 4 1BB ligand receptor antibody
  • 4-1BB ligand receptor antibody
  more

• 应用

  WB: 1:500
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of CD137 on HepG2 cell lysate using anti-CD137 antibody at 1/500 dilution.

   

  

  Fig2: ICC staining CD137 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining CD137 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining CD137 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-CD137 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD137 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD137 antibody. Counter stained with hematoxylin.

   

  

  Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.

   

  

  Fig10: Flow cytometric analysis of Jurkat cells with CD137 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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