Anti-B4GALNT1/GM2 synthase antibody
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概述
- 产品描述Involved in the biosynthesis of gangliosides GM2, GD2 and GA2. Beta-1,4 N-acetylgalactosaminyltransferase 1 is an enzyme that in humans is encoded by the B4GALNT1 gene. GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.
- 产品名称Anti-B4GALNT1/GM2 synthase antibody
- 分子量59 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Rabbit,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human B4GALNT1/GM2 synthase 451-533/533
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A.
- 亚细胞定位Golgi apparatus membrane; Single-pass type II membrane protein.
- 其它名称(N acetylneuraminyl) galactosylglucosylceramide
(N-acetylneuraminyl)-galactosylglucosylceramide
4 N-acetylgalactosaminyltransferase 1
4933429D13Rik
B4GALNT1
B4GN1_HUMAN
Beta 1 4 N acetyl galactosaminyl transferase 1
Beta 1 4 N acetylgalactosaminyltransferase 1
Beta-1
BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE 1
BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE
Beta1 4GalNAc T
Gal-NAc-T
GALGT
Galgt1
GalNAc T
GalNAc-T
GALNACT
GD2 synthase
Ggm-2
Ggm2
GM2 synthase
GM2/GD2 synthase
SIAT 2
SIAT2
UDP Gal:betaGlcNAc beta 1 4 N acetylgalactosaminyltransferase transferase 1
UDP N acetyl alpha D galactosamine:(N acetylneuraminyl).
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (B4GALNT1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- B4GALNT1/GM2 synthase at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Fig3: Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- B4GALNT1/GM2 synthase at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 53 kD
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