Anti-MLH1 antibody
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概述
- 产品描述MLH1 is a DNA mismatch repair protein that heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, and then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the mismatch strand. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may recruit DNA polymerase III to the site of the MMR. Defects in MLH1 are the cause of hereditary nonpolyposis colorectal cancer type 2 (HNPCC2). Most patients with HNPCC have mutations in either the MLH1 or MSH2 genes (1).
- 产品名称Anti-MLH1 antibody
- 分子量85 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC,IP
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20 ℃. Stable for 12 months from date of receipt
- 存储缓冲液1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Tissue culture supernatant
- 亚细胞定位Nucleus
- 其它名称
- COCA 2 antibody
- COCA2 antibody
- DNA mismatch repair protein Mlh1 antibody
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应用
WB: 1:1,000
IP: 1:10-100
FC: 1:10-100
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Fig1: Western blot analysis on (A) 293, (B) HeLa, (C) A431, and (D) SW480 cell lysates using anti-MLH1.
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