Anti-Phospho-FAK (Ser 722) antibody [4G1]

Anti-Phospho-FAK (Ser 722) antibody [4G1]

• 概述

• 产品描述Activation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phospho-rylation of FAK Tyr 397 decreases during serum starvation, contact inhibition and cell cycle arrest, all conditions under which activating FAK Tyr 407 phosphorylation increases.
• 产品名称Anti-Phospho-FAK (Ser 722) antibody [4G1]
• 分子量125 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Cell junction, Cell membrane, Nucleus
• 其它名称
  • FADK 1 antibody
  • FADK antibody
  • FAK related non kinase polypeptide antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

•  

  

  Fig1: A.Western blot analysis of FAK phosphorylation in non-transfected:(A,D), untreated human FAK transfected:(B,E) and lambda protein phosphatase treated human FAK transfected:(C,F) 293T whole cell lysates. Antibodies tested include p-FAK (A-12):(A,B,C) and FAK (C-903):(D,E,F).

   

  

  Fig2: B.Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！