Anti-COX2 antibody
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概述
- 产品描述Prostaglandins are a diverse group of autocrine and paracrine hormones thatmediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) isan intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1(Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyzethe formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 areisozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutivelyexpressed in most tissues and is thought to serve in general “housekeeping”functions. Cox-2 is efficiently induced in migratory cells respondingto pro-inflammatory stimuli and is considered to be an important mediator ofinflammation. Both enzymes are targets for the nonsteroidal therapeutic antiinflammatorydrugs, NSAIDs.
- 产品名称Anti-COX2 antibody
- 分子量70-72kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF,IHC-P,ELISA
- 抗体类型小鼠单抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Microsome membrane, Endoplasmic reticulum membrane
- 其它名称
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
more
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应用
WB: 1:100-1:1000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
ELISA: 1:30-1:3000
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Fig1: Western blot analysis of Cox-2 expression in untreated (A), LPS treated (B) and LPS and Hydrocortisone treated (C) RAW 264.7 whole cell lysates. Note decreased LPS induction of Cox-2 expression in lane C.expression in lane C.
Fig2: Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane and nuclear envelope localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells (B).
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