Anti-PLA2G12A antibody [C11-H2]

Anti-PLA2G12A antibody [C11-H2]

• 概述

• 产品描述Secreted phospholipases A2 (sPLA2s) form a large family of structurally related enzymes, which are widespread in nature. Snake venoms have been known for decades to contain a tremendous molecular diversity of sPLA2s, which can exert a myriad of toxic and pharmacological effects. Secreted phospholipase A2 enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as group XIIA sPLA2, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Group XIIA sPLA2, also known as GXII, ROSSY, PLA2G12 or PLA2G12A, is a 189 amino acid secreted protein that localizes to the cytoplasm. Belonging to the phospholipase A2 family, group XIIA sPLA2 is abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas
• 产品名称Anti-PLA2G12A antibody [C11-H2]
• 分子量21 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted. Cytoplasm
• 其它名称
  • EC 3.1.1.4 antibody
  • Group XII secreted phospholipase A2 antibody
  • Group XIIA secreted phospholipase A2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PLA2G12A on human PLA2G12A recombinant protein using anti-PLA2G12A antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PLA2G12A on HEK293 (1) and PLA2G12A-hIgGFc transfected HEK293 (2) cell lysate using anti-PLA2G12A antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PLA2G12A antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with PLA2G12A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！