Anti-Vimentin antibody [A6-C1]

Anti-Vimentin antibody [A6-C1]

• 概述

• 产品描述Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.
• 产品名称Anti-Vimentin antibody [A6-C1]
• 分子量54 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB, ICC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm
• 其它名称
  • CTRCT30 antibody
  • Epididymis luminal protein 113 antibody
  • FLJ36605 antibody
  more

• 应用

  WB: 1:2,000-1:5,000
  ICC: 1:100-1:200

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  Fig1: Western blot analysis on A431 cell lysates using anti- Vimentin mouse mAb in different dilution.

   

  

  Fig2: ICC staining Vimentin in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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