Anti-SLC32A1 antibody
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概述
- 产品描述The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles. The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS. Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity.
- 产品名称Anti-SLC32A1 antibody
- 分子量57 KDa
- 种属反应性Human,Mouse,Rat,Pig,Cow,Rabbit
- 验证应用WB,IHC-P,ICC/IF
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human SLC30A9 351-450/525
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasmic vesicle membrane.
- 其它名称bA122O1.1
GABA and glycine transporter
hVIAAT
SLC32A 1
Slc32a1
solute carrier family 32 (GABA vesicular transporter) member 1
Solute carrier family 32 member 1
Vesicular GABA Amino Acid Transporter
Vesicular GABA transporter
Vesicular inhibitory amino acid transporter
VGAT
VIAAT
VIAAT_HUMAN.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
IHC-F:1:400-800
IF:1:100-500
ICC/IF:1:100-500
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Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SLC32A1 Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 57kD
Fig3: Sample:heart (Mouse) Lysate at 40 ug
Primary: Anti-SLC32A1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 57kD
Observed band size: 60kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
