Anti-C7 antibody [15D1]

Anti-C7 antibody [15D1]

• 概述

• 产品描述This gene encodes a serum glycoprotein that forms a membrane attack complex together with complement components C5b, C6, C8, and C9 as part of the terminal complement pathway of the innate immune system. Its primary task is to bind the C5bC6 complex together. This junction alters the configuration of the protein molecules, exposing a hydrophobic site on C7 that allows the C7 to insert into the phospholipid bilayer of the pathogen. The protein encoded by this gene contains a cholesterol-dependent cytolysin/membrane attack complex/perforin-like (CDC/MACPF) domain and belongs to a large family of structurally related molecules that form pores involved in host immunity and bacterial pathogenesis. This protein initiates membrane attack complex formation by binding the C5b-C6 subcomplex and inserts into the phospholipid bilayer, serving as a membrane anchor. Mutations in this gene are associated with a rare disorder called C7 deficiency.
• 产品名称Anti-C7 antibody [15D1]
• 分子量94 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide within human aa 450-680.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted.
• 其它名称
  • C7 antibody
  • CO7_HUMAN antibody
  • complement component 7 antibody
  more

• 应用

  ELISA:
  ELISA(Cap):
  WB:1:500-1:2,000
  IP:
  ICC:1:50-1:200
  IF:
  ICC/IF:
  IHC-P:
  IHC-P:
  IHC-F:
  FC:1:50-1:100
  Dot Blot:
  ChIP:
  Immunohistochemistry:

•  

  

  Fig1: Western blot analysis of C7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: HepG2 cell lysate
  Lane 2: K562 cell lysate
  Lane 3: HUVEC cell lysate

   

  

  Fig2: ICC staining of C7 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig3: ICC staining of C7 in HCT116 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody  for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig4: ICC staining of C7 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibodyfor 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig5: Flow cytometric analysis of C7 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody  (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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