Anti-POLR3G antibody
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概述
- 产品描述DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Belongs to the eukaryotic RPC7 RNA polymerase subunit family. subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex.
- 产品名称Anti-POLR3G antibody
- 分子量26 kDa
- 种属反应性Human, Mouse
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from the Internal region of human POLR3G. AA range:151-200
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称
- POLR3G antibody; DNA-directed RNA polymerase III subunit RPC7 antibody; RNA polymerase III subunit C7 antibody; DNA-directed RNA polymerase III subunit G antibody; RNA polymerase III 32 kDa subunit antibody; RPC32 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of PC12 cells using POLR3G Polyclonal Antibody.. Secondary antibody(catalog#:HA1001) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Fig2: Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
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