Anti-NF-κB p105/p50 antibody [A2-7]

Anti-NF-κB p105/p50 antibody [A2-7]

• 概述

• 产品描述NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators.
• 产品名称Anti-NF-κB p105/p50 antibody [A2-7]
• 分子量50kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to NF-κB p105/p50.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm.
• 其它名称
  • DKFZp686C01211 antibody
  • DNA binding factor KBF1 antibody
  • DNA binding factor KBF1 EBP1 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:200
  IHC-P: 1:200-1:500

•  

  

  Fig1: Western blot analysis of NF-κB p105/p50 on MCF-7 cell lysates.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 antibody. Counter stained with hematoxylin.

   

  

  Fig3: ICC staining NF-κB p105/p50 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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