Anti-Connexin 43 antibody
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概述
- 产品描述The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.
- 产品名称Anti-Connexin 43 antibody
- 分子量43 kDa
- 种属反应性Human,Mouse
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within C-terminal human Connexin 43.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Plasma membrane. Endoplasmic reticulum. Gap junction.
- 其它名称Connexin 43 antibody
Connexin-43 antibody
Cx 43 antibody
Cx43 antibody
CXA1_HUMAN antibody
DFNB38 antibody
Gap junction 43 kDa heart protein antibody
Gap junction alpha-1 protein antibody
Gap junction protein alpha 1 43kDa (connexin 43) antibody
Gap junction protein alpha 1 43kDa antibody
Gap junction protein alpha like antibody
GJA 1 antibody
Gja1 antibody
GJAL antibody
ODD antibody
ODDD antibody
ODOD antibody
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应用
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Fig1: Western blot analysis of Connexin 43 on mouse testis tissue lysate using anti-Connexin 43 antibody at 1/2,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Connexin 43 antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of SH-SY-5Y cells with Connexin 43 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
