Anti-CK5 antibody [6D-4D]

Anti-CK5 antibody [6D-4D]

• 概述

• 产品描述Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB).
• 产品名称Anti-CK5 antibody [6D-4D]
• 分子量62 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • 58 kDa cytokeratin antibody
  • CK-5 antibody
  • CK5 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of CK5 on different cells lysates using anti-CK5 antibody at 1/1,000 dilution.
  Positive control:
  Line1: A431
  Line2: 3T3-L1
  Line3: COS7
  Line4: MCF-7
  Line5: SK-Br-3
  Line6: Hela
  Line7: Lncap
  Line8: HepG2

   

  

  Fig2: Western blot analysis of CK5 on human CK5 recombinant protein using anti-CK5 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CK5 on HEK293 (1) and CK5-hIgGFc transfected HEK293 (2) cell lysate using anti-CK5 antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining CK5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- CK5 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with CK5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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