Anti-TRIM28 antibody

Anti-TRIM28 antibody

• 概述

• 产品描述TRIM28, also known as transcriptional intermediary factor 1β (TIF1β) and KAP1, is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TIF1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors.
• 产品名称Anti-TRIM28 antibody
• 分子量110 kDa

• 种属反应性Human,Mouse

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within human TRIM28 400-500 aa.

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Nucleus

   

• 其它名称

  more

  • E3 SUMO protein ligase TRIM28 antibody

  • E3 SUMO-protein ligase TRIM28 antibody

  • FLJ29029 antibody

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of TRIM28 on different cell lysates using anti-TRIM28 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining TRIM28 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.

   

  

  Fig9: Flow cytometric analysis of Hela cells with TRIM28 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary

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