Anti-RALB antibody [B4-F8]

Anti-RALB antibody [B4-F8]

• 概述

• 产品描述Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A.
• 产品名称Anti-RALB antibody [B4-F8]
• 分子量23 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • 5730472O18Rik antibody
  • dRalb antibody
  • GTP binding protein antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of RALB on human RALB recombinant protein using anti-RALB antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of RALB on HEK293 (1) and RALB-hIgGFc transfected HEK293 (2) cell lysate using anti-RALB antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of RALB on different cell lysate using anti-RALB antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HepG2
  Lane 2: COS7
  Lane 3:NIH/3T3
  Lane 4: A549
  Lane 5: U251
  Lane 6:HT-29
  Lane 7: HEK293
  Lane 8: LOVO

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-RALB antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using anti-RALB antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with RALB antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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