Anti-GFPT1 antibody
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概述
- 产品描述Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1. Defects in GFPT1 are the cause of limb-girdle myasthenia with tubular aggregates (LGMTA). A congenital myasthenic syndrome characterized by onset of proximal muscle weakness in the first decade.
- 产品名称Anti-GFPT1 antibody
- 分子量79 kDa
- 种属反应性Human,Monkey,Rat
- 验证应用WB,ICC,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Cytosol. Extracellular exosome.
- 其它名称
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:100-1:200
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Fig1: Western blot analysis of GFPT1 on human GFPT1 recombinant protein using anti-GFPT1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of GFPT1 on HEK293 (1) and GFPT1-hIgGFc transfected HEK293 (2) cell lysate using anti-GFPT1 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of GFPT1 on different cell lysate using anti-GFPT1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: HEK293
Lane 3: BEL-7402
Lane 4: SMMC-7721
Lane 5: SK-MES-1
Lane 6: C6
Lane 7: COS7
Fig4: ICC staining GFPT1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with GFPT1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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