Anti-POLR3C antibody
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概述
- 产品描述DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
- 产品名称Anti-POLR3C antibody
- 分子量61 KDa
- 种属反应性Human
- 验证应用WB,IHC-P,ICC/IF
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human POLR3C 21-120/534
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus.
- 其它名称POLR3C
DNA directed III 62 kDa polypeptide
DNA-directed RNA polymerase III subunit C
DNA-directed RNA polymerase III subunit rpc3
Polymerase (RNA) III (DNA directed) polypeptide C (62kD)
RNA polymerase C subunit 3
RNA polymerase III 62 kDa subunit
RNA polymerase III C62 subunit
RNA polymerase III subunit C3
RPC3
RPC3_HUMAN.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
IHC-F:1:400-800
IF:1:100-500
ICC/IF:1:100-500
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Fig1: Tissue/cell: human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-POLR3C Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Protein:
Raji(human) lysate at 40ug;
U937(human) lysate at 40ug;
Primary: rabbit Anti-POLR3Cat 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
