Anti-WHSC2 antibody [H8-B11]

Anti-WHSC2 antibody [H8-B11] 

• 概述

• 产品描述NELF-A, for negative elongation factor A, is a protein factor required for DRB-sensitive transcription. NELF-A is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF. DSIF and NELF act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT. NELF-A is also known as WHSC2 (Wolf-Hirschhorn syndrome candidate 2). Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). The human NELF-A gene maps to chromosome 4p16.3 and encodes a 528 amino acid protein that is expressed in endothelial cells.
• 产品名称Anti-WHSC2 antibody [H8-B11]
• 分子量57 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • FLJ10442 antibody
  • FLJ25112 antibody
  • Negative elongation factor A antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of WHSC2 on HEK293 (1) and WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.
  Positive control:
  Line1:Jurkat Line2:HeLa Line3:HEK293
  Line4:Rat brain Line5:A549 Line6:SPC-A-1

   

  

  Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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