Anti-DDX20 antibody [F9-6C]

Anti-DDX20 antibody [F9-6C]

• 概述

• 产品描述Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2. It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. It is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies). Gemin3 also interacts with SmB, SmD2 and SmD3. It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins. Gemin3 is a putative RNA helicase and shows ATPase activity. It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung.
• 产品名称Anti-DDX20 antibody [F9-6C]
• 分子量92 kDa
• 种属反应性Rat,Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleu
• 其它名称
  • Component of gems 3 antibody
  • DDX 20 antibody
  • ddx20 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of Gemin 3 on human Germin 3 recombinant protein using anti-Germin 3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Germin 3 on HEK293 (1) and Germin 3-hIgGFc transfected HEK293 (2) cell lysat using anti- Germin 3 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of Germin 3 on different cell lysat using anti- Germin 3 antibody at 1/1,000 dilution.
  Positive control: Line1: Jurkat Line2: Hela Line3: NTERA-2 Line4: HL7702 Line5:K562 Line6:C6

   

  

  Fig4: ICC staining Germin 3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of Hela cells with Germin 3 antibody at 1/50 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！