Anti-CD96 antibody [5E6C12]

Anti-CD96 antibody [5E6C12]

• 概述

• 产品描述CD96 (Cluster of Differentiation 96) or Tactile (T cell activation, increased late expression) is a receptor expressed on T cells and NK cells, and shares sequence similarity with CD226 (also known as DNAM-1). The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesion of activated T and NK cells to their target cells during the late phase of the immune response. It may also function in antigen presentation[citation needed]. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. CD96 is a transmembrane glycoprotein that has three extracellular immunoglobulin-like domains and is expressed by all resting human and mouse NK cells. CD96 main ligand is CD155. CD 96 has approximately 20% homology with CD226 and competed for binding to CD155 with CD226.
• 产品名称Anti-CD96 antibody [5E6C12]
• 分子量65.6kDa
• 种属反应性Human,Mouse
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human CD96 (AA: extra 321-519) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG2b
• 纯化方式Protein G purified.
• 亚细胞定位Membrane.
• 其它名称
  • CD96 antibody
  • CD96 molecule antibody
  • Cell surface antigen CD96 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

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  Fig1: Western blot analysis of CD96 against human CD96 (AA: extra 321-519) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of against HEK293 (1) and CD96 (AA: extra 321-519)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Western blot analysis of 1 againstHela (1), Jurkat (2), MOLT4 (3), Raji (4), HL-60 (5), U937 (6), and C6 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig4: Flow cytometric analysis of CD96 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody ((green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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