Anti-NFIB NF1B2 antibody [1H2]

Anti-NFIB NF1B2 antibody [1H2]

• 概述

• 产品描述NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
• 产品名称Anti-NFIB NF1B2 antibody [1H2]
• 分子量47 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant full length protein corresponding to human NFIB/NF1B2.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein G purified.
• 亚细胞定位Nucleus.
  
• 其它名称
  • CCAAT Box Binding Transcription Factor antibody
  • CCAAT-box-binding transcription factor antibody
  • CTF antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Flow cytometric analysis of SH-SY-5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the s

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