Anti-BHMT antibody [9E1]

Anti-BHMT antibody [9E1]

• 概述

• 产品描述Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.
• 产品名称C [9E1]
• 分子量45 kDa
• 种属反应性Human,Mouse,Rat
• 抗体类型小鼠单抗
• 免疫原Recombinant protein.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • Betaine homocysteine methyltransferase antibody
  • Betaine homocysteine S methyltransferase 1 antibody
  • Betaine homocysteine S methyltransferase antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:50
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining BHMT (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-BHMT antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of HepG2 cells with BHMT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG1 was used as the secondary

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