Anti-Smad2 antibody [C9-B0]

Anti-Smad2 antibody [C9-B0]

• 概述

• 产品描述Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
• 产品名称Anti-Smad2 antibody [C9-B0]
• 分子量58 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • Drosophila, homolog of, MADR2 antibody
  • hMAD-2 antibody
  • HsMAD2 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of Smad2 on Hela cell lysate using anti-Smad2 antibody at 1/500 dilution.

   

  

  Fig2: ICC staining Smad2 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Smad2 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining Smad2 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Smad2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Smad2 antibody. Counter stained with hematoxylin.

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