Anti-AK6 antibody [B1-F4]

Anti-AK6 antibody [B1-F4]

• 概述

• 产品描述In eukaryotic systems, the process of initiating transcription from protein-coding genes requires the presence of RNA polymerase II and a broad family of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that mediate the transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. TFIID, one of the basal transcription factors, facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element. TAF9 (transcription initiation factor TFIID subunit 9), also known as TAF9A or TAF II p32, is a 264 amino acid nuclear protein that, is a component of the TFIID complex and is required for cell viability.
• 产品名称Anti-AK6 antibody [B1-F4]
• 分子量20 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • AD-004 antibody
  • Adenylate kinase isoenzyme 6 antibody
  • Adrenal gland protein AD-004 antibody
  more

• 应用

  WB: 1:2,000-1:5,000
  ICC: 1:50-1:200
  FC: 1:50-1:00

•  

  

  Fig1: Western blot analysis of AK6 on MCF-7 cell (1) and human lung tissue (2) lysate using anti-AK6 antibody at 1/1000 dilution.

   

  

  Fig2: ICC staining AK6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Flow cytometric analysis of MCF-7 cells with AK6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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