Anti-eIF4A1 antibody [M8]

Anti-eIF4A1 antibody [M8]

• 概述

• 产品描述ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. Belongs to the DEAD box helicase family. eIF4A subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. subunit:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and RENT2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1.
• 产品名称Anti-eIF4A1 antibody [M8]
• 分子量48 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, IHC-P, IF
• 抗体类型小鼠单抗
• 免疫原Synthetic Peptide of eIF4A1
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• 亚型IgG1
• 纯化方式Immunogen affinity purified.
• 亚细胞定位Cytosol, Extracellular region or secreted.
• 其它名称
  • ATP-dependent RNA helicase eIF4A-1 antibody
  • DDX2 antibody
  • DDX2A antibody
  more

• 应用

  WB: 1:1000-3000
  IF: 1:100-200
  IHC-P: 1:50-300

•  

  

  Fig1: Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

   

  

  Fig2: Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

   

  

  Fig3: Western blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse Brain tissue,

   

  

  Fig4: IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

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