Anti-Podoplanin antibody
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概述
- 产品描述May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
- 产品名称Anti-Podoplanin antibody
- 分子量15 KDa
- 种属反应性Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from mouse Podoplanin 91-166/166
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Membrane; Plasma membrane.
- 其它名称Podoplanin /gp36
Podoplanin Protein
AGGRUS
GLYCOPROTEIN 36 KD
Podoplanin
Glycoprotein 36
gp 36
GP 38
GP 40
gp36
GP38
GP40
HT1A 1
HT1A1
hT1alpha1
hT1alpha2
Lung type I cell membrane associated glycoprotein
Lung type I cell membrane associated glycoprotein isoform a
Lung type I cell membrane associated glycoprotein T1A 2
OTS 8
OTS8
OTTHUMP00000009640
OTTHUMP00000044504
PA2.26
PA2.26 antigen
PDPN
Podoplanin
T1 alpha
T1 ALPHA GENE
T1A
TIA 2
TIA2.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- Podoplanin at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 37 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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