Anti-Bmi1 antibody [B3-G5]

Anti-Bmi1 antibody [B3-G5]

• 概述

• 产品描述The Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest.
• 产品名称买二赠二Anti-Bmi1 antibody [B3-G5]
• 分子量37kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human Bmi1 full sequence.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus, Cytoplasm
  
• 其它名称
  • B lymphoma Mo MLV insertion region (mouse) antibody
  • B lymphoma Mo MLV insertion region 1 homolog antibody
  • Bmi 1 antibody
  more

• 应用

  WB: 1:1,000
  IHC-P: 1:200
  ICC: 1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: 293T
  Lane 2: Jurkat
  Lane 3: Hela
  Lane 4: MCF-7
  Lane 5: HepG2
  Lane 6: NIH/3T3
  Lane 7: PC12
  Lane 8: Mouse kidney
  Lane 9: Human kidney
  Lane 10: K562
  Lane 11: Human brain

   

  

  Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

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