Anti-AKR1C1 antibody [5-F10]

Anti-AKR1C1 antibody [5-F10] 

• 概述

• 产品描述Human liver contains isoforms of dihydrodiol dehydrogenase (DD1, DD2, DD3 and DD4), which belong to the aldo-oxo reductase / aldo-keto reductase (AKR) superfamily, have 20α- or 3α-hydroxysteroid dehydrogenase AKD1C3 and 3α-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 is also designated as AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C4, CD, CHDR or AKR1C6 (in rodents). DD1 and DD2 are 20α-HSDs, DD3 and DD4 are the 3α-HSDs. The 20α-HSD catalyzes the reaction of progesterone to the inactive form 20α-hydroxyprogesterone. The 3α-HSD is a cytosolic, monomeric, NADPH-depen DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver. DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzene dihydrodiol and trans- naphthalenedihydrodiol.
• 产品名称Anti-AKR1C1 antibody [5-F10]
• 分子量37 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • 2-dihydrobenzene-1 antibody
  • 2-diol dehydrogenase antibody
  • 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
  more

• 应用

  WB: 1:2,000-1:10,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution.
  Positive control:
  Lane 1: HepG2
  Lane 2: Human liver tissue

   

  

  Fig2: ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！