Anti-CD45 antibody
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概述
- 产品描述The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
- 产品名称Anti-CD45 antibody
- 分子量Predicted band size 147 kDa.
- 种属反应性Human
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human CD45 aa 1-60.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cell membrane, membrane raft.
- 其它名称
- B220 antibody
- CD 45 antibody
- CD45 antibody
more
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应用
WB:1:500-1:1,000
FC:1:50-1:100
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Fig1: Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody.
Lane 1: Anti-CD45 Antibody (1/500).
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
Fig2: Western blot analysis of CD45 on Jurkat cell lysates using anti-CD45 antibody.
Lane 1: Anti-CD45 Antibody (1/500).
Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide.
Fig3: Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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