Anti-PTEN antibody
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概述
- 产品描述As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers. PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm and shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells.
- 产品名称Anti-PTEN antibody
- 分子量47 kDa
- 种属反应性Human, Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human PTEN aa 50-250.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus. Cytoplasm. Secreted.
- 其它名称10q23del antibody
BZS antibody
DEC antibody
GLM2 antibody
MGC11227 antibody
MHAM antibody
MMAC1 antibody
MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody
Mutated in multiple advanced cancers 1 antibody
Phosphatase and tensin homolog antibody
Phosphatase and tensin like protein antibody
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody
Pten antibody
PTEN_HUMAN antibody
PTEN1 antibody
TEP1 antibody
more
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应用
WB: 1:500
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of PTEN on MCF-7 cell lysates using anti-PTEN antibody at 1/500 dilution.
Fig2: ICC staining PTEN in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PTEN in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining PTEN in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PTEN antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-PTEN antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PTEN antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of SH-SY5Y cells with PTEN antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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