Anti-CCL2/MCP1 antibody

Anti-CCL2/MCP1 antibody

• 概述

• 产品描述Acts as a ligand for C-C chemokine receptor CCR2 . Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level).
• 产品名称Anti-CCL2/MCP1 antibody
• 分子量Predicted band size: 11 kDa.
• 种属反应性Human
• 验证应用WB,IHC-P,ICC
• 抗体类型兔多抗
• 免疫原Synthetic peptide within human CCL2/MCP1 aa 50-99.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified.
• 亚细胞定位Secreted.
• 数据链接SwissProt: P13500 Human
  
• 其它名称
  • C-C motif chemokine 2 antibody
  • CCL2 antibody
  • CCL2_HUMAN antibody
  more

• 应用

  WB: 1:500
  IHC-P: 1:100-1:500
  ICC: 1:50-1:200

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  Fig1: Western blot analysis of CCL2/MCP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: A549 cell lysate
  Lane 2: Hela cell lysate

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CCL2/MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CCL2/MCP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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