Anti-MAP3K13/LZK antibody

Anti-MAP3K13/LZK antibody

• 概述

• 产品描述Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.
• 产品名称Anti-MAP3K13/LZK antibody
• 分子量108 KDa
• 种属反应性Human,Mouse,Cow,Orangutan
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human MAP3K13/LZK 801-900/966
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm. Membrane.
• 其它名称EC 2.7.11.25
  Leucine zipper bearing kinase
  Leucine zipper bearing kinase
  Leucine zipper-bearing kinase
  LZK
  M3K13_HUMAN
  MAP3K13
  MEKK13
  MGC133196
  Mitogen activated protein kinase kinase kinase 13
  Mitogen-activated protein kinase kinase kinase 13
  Mixed lineage kinase
  MLK
  OTTHUMP00000210397.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample: K562 Cell (Human) Lysate at 40 ug
  Primary: Anti-MAP3K13/LZKat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 108 kD
  Observed band size: 108 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugatedat 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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