Anti-CLOCK antibody
-
概述
- 产品描述Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the Clock gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that Clock may regulate circadian rhythmicity in combination with other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye.
- 产品名称Anti-CLOCK antibody
- 分子量95kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm, Nucleus
- 其它名称
- bHLHe8 antibody
- Circadian locomoter output cycles kaput protein antibody
- Circadian locomoter output cycles protein kaput antibody
more
-
应用
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis on different lysates using anti-CLOCK rabbit polyclonal antibody. Positive control:
Lane 1: PC-12
Lane 2: NIH/3T3
Lane 3: Hela
Lane 4: MCF-7
Lane 5: Mouse brain
Lane 6: Mouse kidney
Fig2: Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody.
Fig4: Immunocytochemical staining of Hela cells using anti-CLOCK rabbit polyclonal antibody.
Fig5: Immunocytochemical staining of 293 cells using anti-CLOCK rabbit polyclonal antibody.
Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-CLOCK rabbit polyclonal antibody.
Fig7: Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CLOCK rabbit polyclonal antibody.
Fig8: Immunohistochemical analysis of paraffin- embedded mouse skeletal muscle tissue using anti-CLOCK rabbit polyclonal antibody.
Fig9: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-CLOCK rabbit polyclonal antibody.
Fig10: Flow cytometric analysis of Hela cells with CLOCK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
