Anti-Cytochrome P450 2E1 antibody
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概述
- 产品描述Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
- 产品名称Anti-Cytochrome P450 2E1 antibody
- 分子量57 kDa
- 种属反应性Human, Mouse
- 验证应用WB, ICC, IHC-P
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human Cytochrome P450 2E1 aa 406-449.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Endoplasmic reticulum membrane. Microsome membrane.
- 其它名称
- 4-nitrophenol 2-hydroxylase antibody
- CP2E1_HUMAN antibody
- CPE1 antibody
more
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应用
WB: 1:1000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of Cytochrome P450 2E1 on different lysates using anti-Cytochrome P450 2E1 antibody at 1/1000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: NIH-3T3
Lane 3: Mouse kidney tissue
Lane 4: siHa
Fig2: ICC staining Cytochrome P450 2E1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Cytochrome P450 2E1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytochrome P450 2E1 antibody. Counter stained with hematoxylin.
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