Anti-GPR107 antibody

Anti-GPR107 antibody

• 概述

• 产品描述GPR107 is a G-protein coupled receptor which contains a carboxy-terminal seven transmembrane domain (LUSTR domain) similar to GPCRs. Three named isoforms exist.
• 产品名称Anti-GPR107 antibody
• 分子量63 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Horse,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human GPR107 301-400/600
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; integral to membrane.
• 其它名称bA138E2.2
  DKFZp667C222
  FLJ16312
  FLJ20998
  FLJ22591
  GP107_HUMAN
  G protein coupled receptor 107
  GCDRP
  GPR 107
  KIAA1624
  Lung seven transmembrane receptor 1
  LUSTR1
  MGC126118
  MGC15440
  protein GPR107
  RP11 88G17.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample: A549 Cell (Human) Lysate at 40 ug
  Primary: Anti-GPR107  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 63 kD
  Observed band size: 63 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GPR107) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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