Anti-Thioredoxin antibody
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概述
- 产品描述Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
- 产品名称Anti-Thioredoxin antibody
- 分子量12 kDa, additional band 40 kDa.
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Nucleus. Cytoplasm. Secreted.
- 其它名称
- ADF antibody
- ATL-derived factor antibody
- SASP antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:100
FC: 1:50-1:100
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Fig1: Western blot analysis of Thioredoxin on different cell lysates using anti-Thioredoxin antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: A431
Lane 4: PC-3M
Fig2: ICC staining Thioredoxin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Thioredoxin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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