Anti-HAO1 antibody
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概述
- 产品描述Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
- 产品名称Anti-HAO1 antibody
- 分子量41 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human GOX/HAO1 261-370/370
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Peroxisome.
- 其它名称Glycolate oxidase
GOX
GOX1
HAO1
HAOX1
HAOX1_HUMAN
Hydroxyacid oxidase 1
MGC142225
MGC142227
OTTHUMP00000030231
(S) 2 hydroxy acid oxidase.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HAO1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-HAO1at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!