Anti-Metadherin(LYRIC) antibody
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概述
- 产品描述MTDH (metastasis adhesion protein), also known as astrocyte elevated gene-1 protein (AEG1) or lysine-rich CEACAM1 co-isolated protein (LYRIC), is a 582 amino acid single-pass membrane protein. Overexpressed in various cancers, MTDH activates the transcription factor NFκB and promotes anchorage-independent growth of immortalized astrocytes and melanocytes, which are key components of tumor growth. MTDH is also thought to enhance the seeding of tumor cells to the target organ endothelium. Localized mainly to the endoplasmic reticulum membrane, MTDH also translocates from the cytoplasm to the nucleus when induced by TNFα. MTDH also localizes to tight junctions (TJ) during the maturation of TJ complexes in epithelial cells. MTDH is found at highest levels in heart, skeletal muscle, tongue and small intestine.
- 产品名称Anti-Metadherin(LYRIC) antibody
- 分子量64 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Endoplasmic reticulum membrane. Nucleus.
- 其它名称
- 3D3 antibody
- 3D3/LYRIC antibody
- AEG 1 antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:100-1:500
FC: 1:50-1:200
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Fig1: Western blot analysis of Metadherin(LYRIC) on human skeletal muscle tissue (1) and MCF-7 cell (2) lysate using anti-Metadherin(LYRIC) antibody at 1/1,000 dilution.
Fig2: ICC staining Metadherin(LYRIC) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Metadherin(LYRIC) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Metadherin(LYRIC) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Metadherin(LYRIC) antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY5Y cells with Metadherin(LYRIC) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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