Anti-Restriction enzyme FokI(C-term) antibody

Anti-Restriction enzyme FokI(C-term) antibody

• 概述

• 产品描述The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
• 产品名称Anti-Restriction enzyme FokI(C-term) antibody
• 分子量66kDa
• 种属反应性Planomicrobium okeanokoites
• 验证应用WB
• 抗体类型兔多抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Immunogen affinity purified
• 亚细胞定位--

• 应用

  WB: 1:1000-1:2000

•  

  

  Fig1: Western blot analysis on purified restriction enzyme FokI.

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