Anti-Prohibitin（Mitochondrial Marker） antibody

Anti-Prohibitin（Mitochondrial Marker） antibody

• 概述

• 产品描述Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed.
• 产品名称Anti-Prohibitin（Mitochondrial Marker） antibody
• 分子量30kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Mitochondrion inner membrane

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• 其它名称

  more

  • Epididymis luminal protein 215 antibody

  • Epididymis secretory sperm binding protein Li 54e antibody

  • HEL 215 antibody

• 应用

  WB: 1:1,000-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:10-1:100

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  Fig1: Western blot analysis on different cell lysates using anti-Prohibitin rabbit polyclonal antibody. Positive control:
  Lane 1: F9
  Lane 2: Jurkat
  Lane 3: Mouse kidney
  Lane 4: PC-12

   

  

  Fig2: Immunocytochemical staining of Hela cells using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig3: Immunocytochemical staining of HepG2 cells using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig4: Immunocytochemical staining of C2C12 cells using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig5: Immunohistochemical analysis of paraffin- embedded human liver carcinoma tissue using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig7: Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig8: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig9: Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Prohibitin rabbit polyclonal antibody.

   

  

  Fig10: Flow cytometric analysis of Hela cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secon

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