Anti-SMAD3 antibody

Anti-SMAD3 antibody

• 概述

• 产品描述Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
• 产品名称Anti-SMAD3 antibody
• 分子量48 kDa

• 种属反应性Human,Mouse,Rat,Zebrafish

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoplasm, Nucleus, endoplasmic reticulum

•  

• 其它名称

  more

  • DKFZP586N0721 antibody

  • DKFZp686J10186 antibody

  • hMAD 3 antibody

• 应用

  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100
  WB: 1:500-1:1,000

•  

  

  Fig1: Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig2: Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig3: Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig4: Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig5: Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig6: Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig7: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig8: Immunohistochemical analysis of paraffin- embedded human stomach carcinoma tissue using anti-SMAD3 rabbit polyclonal antibody. .

   

  

  Fig9: Flow cytometric analysis of Hela cells with SMAD3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary a

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！