Anti-Ub antibody
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概述
- 产品描述Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
- 产品名称Anti-Ub antibody
- 分子量80 50 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用IF, WB, IHC-P
- 抗体类型兔多抗
- 免疫原Synthesized peptide derived from the N-terminal region of human Ub.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 其它名称
- UBB antibody; Polyubiquitin-B antibody; UBC antibody; Polyubiquitin-C antibody; RPS27A antibody; UBA80 antibody; UBCEP1 antibody; Ubiquitin-40S ribosomal protein S27a antibody; Ubiquitin carboxyl extension protein 80 antibody; UBA52 antibody; UBCEP2 antibody; Ubiquitin-60S ribosomal protein L40 antibody; CEP52 antibody; Ubiquitin A-52 residue ribosomal protein fusion product 1 antibody;
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应用
IF: 1:50-200 WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Immunofluorescence analysis of human-lung tissue. 1,Ub Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Fig2: Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Ub Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Fig3: Western Blot analysis of K562, L929 cells using Ub Polyclonal Antibody.. Secondary antibody（catalog#：HA1001) was diluted at 1:20000
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
