Anti-Adenosine A3 Receptor antibody
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概述
- 产品描述Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.
- 产品名称Anti-Adenosine A3 Receptor antibody
- 分子量35 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ADORA3 85-180/347
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane.
- 其它名称adenosine A3 receptor
A3AR
AD 026
AD026
ADORA 3
ADORA3
bA552M11.5
RP11 552M11.7
TGPCR1
adenosine receptor A3 isoform 1
adenosine receptor A3 isoform 2
AA3R_HUMAN.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:1μg /test
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Fig1: Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃).
Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody; Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.
Fig2: Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Sample:
Colon carcinoma lysates (Human) Lysate at 40 ug
Gastric carcinoma lysates (Human) Lysate at 40 ug
Primary: Anti- ADORA3 at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
