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Anti-TNF-α antibody
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:50-1:200
Fig1: Western Blot analysis of various cells using TNF-α Polyclonal Antibody. Secondary antibody(catalog#:HA1001) was diluted at 1:20000.
Fig2: Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Fig3: Immunofluorescence analysis of human-stomach tissue. 1,TNF-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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