Anti-eIF3L antibody
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概述
- 产品描述Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,Belongs to the eIF-3 subunit L family. subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
- 产品名称Anti-eIF3L antibody
- 分子量67 kDa
- 种属反应性Human, Mouse
- 验证应用WB, IF
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human IF3EI. AA range:1-50
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称EIF3L antibody; EIF3EIP antibody; EIF3S6IP antibody; HSPC021 antibody; HSPC025 antibody; MSTP005 antibody; Eukaryotic translation initiation factor 3 subunit L antibody; eIF3l antibody; Eukaryotic translation initiation factor 3 subunit 6-interacting protein antibody; Eukaryotic translation initiation factor 3 subunit E antibody;
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应用
WB: 1:500-1:2000
IF: 1:100-1:500
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Fig1: Western Blot analysis of various cells using eIF3L Polyclonal Antibody diluted at 1：1000
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