Anti-TRF2 antibody

Anti-TRF2 antibody

• 概述

• 产品描述Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
• 产品名称Anti-TRF2 antibody
• 分子量55 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human TRF2 301-400/500
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nucleus. Chromosome, telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
• 其它名称Telomeric DNA binding protein
  Telomeric repeat binding factor 2
  Telomeric repeat binding protein 2
  TERF 2
  TERF2
  TRBF 2
  TRBF2
  TRF 2
  TTAGGG repeat binding factor 2.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Lung (Mouse) Lysate at 40 ug
  Primary: Anti-TRF2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

   

  

  Fig2: Sample:
  Brain (Mouse) Lysate at 40 ug
  Primary: Anti-TRF2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Telomeric repeat binding protein 2 ) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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