Anti-ERK 8 antibody
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概述
- 产品描述catalytic activity:ATP + a protein = ADP + a phosphoprotein. domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation. domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1. In vitro, phosphorylates MBP. PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1. tissue specificity:Widely expressed with a maximal expression in lung and kidney.
- 产品名称Anti-ERK 8 antibody
- 分子量60 kDa
- 种属反应性Human, Mouse
- 验证应用WB, IHC-P, IF
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human MAPK15. AA range:141-190
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称MAPK15 antibody; ERK7 antibody; ERK8 antibody; Mitogen-activated protein kinase 15 antibody; MAP kinase 15 antibody; MAPK 15 antibody; Extracellular signal-regulated kinase 7 antibody; ERK-7 antibody; Extracellular signal-regulated kinase 8 antibody; ERK-8 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:100-1:500
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Fig1: Western Blot analysis of various cells using ERK 8 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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