Anti-Cleaved-Caspase-9 (D353) antibody

Anti-Cleaved-Caspase-9 (D353) antibody

• 概述

• 产品描述CASP9 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 9 can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. Caspase 9 is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

• 产品名称Anti-Cleaved-Caspase-9 (D353) antibody

• 分子量17 kDa

• 种属反应性Mouse, Rat

• 验证应用IF, WB, IHC-P

• 抗体类型兔多抗

• 免疫原The antiserum was produced against synthesized peptide derived from human Caspase 9. AA range:323-372

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 亚型IgG

• 纯化方式Immunogen affinity purified.

• 亚细胞定位Cytosol, Mitochondrion, Nucleus.

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• ​其它名称CASP9 antibody; MCH6 antibody; Caspase-9 antibody; CASP-9 antibody; Apoptotic protease Mch-6 antibody; Apoptotic protease-activating factor 3 antibody; APAF-3 antibody; ICE-like apoptotic protease 6 antibody; ICE-LAP6 antibody;

• 应用

  IF: 1:50-200 WB: 1:500-1:2000
  IHC-P: 1:100-1:300

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  Fig1: Immunofluorescence analysis of human-stomach-cancer tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded Human-testis tissue. 1,Cleaved-Caspase-9 (D353) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

   

  

  Fig3: Western Blot analysis of various cells using Cleaved-Caspase-9 (D353) Polyclonal Antibody diluted at 1：1000

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